Document Type
Report
Department
Biological and Biomedical Sciences; Paediatrics and Child Health
Abstract
Numerous safe and effective coronavirus disease 2019 vaccines have been developed worldwide that use various delivery technologies and engineering strategies. We show here that vaccines containing prefusion-stabilizing S mutations elicit antibody responses in humans with enhanced recognition of S and the S1 subunit relative to postfusion S as compared with vaccines lacking these mutations or natural infection. Prefusion S and S1 antibody binding titers positively and equivalently correlated with neutralizing activity, and depletion of S1-directed antibodies completely abrogated plasma neutralizing activity. We show that neutralizing activity is almost entirely directed to the S1 subunit and that variant cross-neutralization is mediated solely by receptor binding domain-specific antibodies. Our data provide a quantitative framework for guiding future S engineering efforts to develop vaccines with higher resilience to the emergence of variants than current technologies.
Publication (Name of Journal)
Science immunology
Recommended Citation
Bowen, J. E.,
Park, Y.,
Stewart, C.,
Brown, J. T.,
Sharkey, W. K.,
Walls, A. C.,
Joshi, A.,
Ahmed, K.,
Shariq, A.,
Iqbal, N. T.
(2022). SARS-CoV-2 spike conformation determines plasma neutralizing activity elicited by a wide panel of human vaccines. Science immunology, 7(78).
Available at:
https://ecommons.aku.edu/pakistan_fhs_mc_women_childhealth_paediatr/1257
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Included in
Emergency Medicine Commons, Infectious Disease Commons, Plasma and Beam Physics Commons, Public Health Commons, Virus Diseases Commons
Comments
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