Comparison of ketamine/xylazine and isoflurane anesthesia on the establishment of mouse middle cerebral artery occlusion model

Authors

Chenyang Gu, Southern Medical University, China
Jiale Liu, Southern Medical University, China
Yajing Li, Southern Medical University, China
Qiankun Zhang, Southern Medical University, China
Chaoqun Lin, University of Chinese Academy of Sciences Shenzhen Hospital, China
Jiajun Huang, Southern Medical University, China
Wenjie Duan, Southern Medical University, China
Yushu Deng, Southern Medical University, China
Waqas Ahmed, Southeast University, China
Ahsan Ali Khan, Aga Khan UniversityFollow

Document Type

Article

Department

Neurosurgery; Surgery

Abstract

The middle cerebral artery occlusion model (MCAO) is one of the most common stroke models in neuroscience research. The establishment of the mouse MCAO model in terms of animal survival depends on anesthesia, which is an important part of the entire surgical process. The 7-day survival rate of the MCAO model under isoflurane (ISO) anesthesia (35%) was lower than ketamine/xylazine (KX) anesthesia (70%), which demonstrated that the success rate of the MCAO model under KX anesthesia would be significantly higher than that under ISO anesthesia. As confirmed by TTC staining and MRI, the cerebral infarction area of mice successfully modeled under ISO anesthesia was significantly smaller than that of KX anesthesia. The diameter of cerebral blood vessels under ISO anesthesia was significantly larger than that under KX, and the blood perfusion volume was also significantly increased in the same area. ISO has proven to delay the coagulation time and affect the activation of coagulation factors. ISO anesthesia may cause bleeding, vasodilation, respiratory depression, and other phenomena that affect the success rate and death of diseased animal models. In conclusion, compared with ISO anesthesia, KX anesthesia is a safer and more suitable method for the establishment of a mouse MCAO model. The data will inform safer and more detailed anesthesia recommendations for the establishment of animal models of vascular-related major injury diseases.

Comments

Volume, issue, and pagination are not provided by the author/publisher

Publication (Name of Journal)

Experimental animals

Recommended Citation

Gu, C., Liu, J., Li, Y., Zhang, Q., Lin, C., Huang, J., Duan, W., Deng, Y., Ahmed, W., Khan, A. A. (2022). Comparison of ketamine/xylazine and isoflurane anesthesia on the establishment of mouse middle cerebral artery occlusion model. Experimental animals.
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_surg_neurosurg/337