Effect of commercial immunoglobulin G preparation on human monocyte Fc-receptor dependent binding of antibody coated platelets
Document Type
Article
Department
Haematology and Oncology, East Africa
Abstract
This study examined the in vitro effect of a commercial immunoglobulin preparation on human monocytes and the Fc-receptor dependent binding of antibody coated platelets. Monocytes were exposed to Sandoglobulin in vitro and subsequently examined for membrane surface bound IgG. Dramatic increments of surface IgG were found which were maximal with 18 h exposure and somewhat higher at 4°C than 37°C. Ultracentrifugations of Sandoglobulin immediately prior to monocyte exposure reduced the monocyte membrane IgG by 75%. The 18 h exposure at 37°C produced dramatic impairment of monocyte Fc-receptor binding of IgG coated platelets (P < 0.001) while exposure for 18 h at 4°C produced a modest impairment of Fc-receptor function. These studies indicate that Sandoglobulin contains IgG aggregates which are able to firmly bind to the monocyte surface in a time and temperature dependent fashion. The dramatic impairment of Fc-receptor function at 37°C and not at 4°C suggests that Fc-receptor modulation, as well as competitive inhibition/steric hindrance, contribute to impairment of monocyte Fc-receptor function.
Publication (Name of Journal)
British Journal of Haematology
DOI
https://doi.org/10.1111/j.1365-2141.1988.tb04178.x
Recommended Citation
Saleh, M.,
Court, W.,
Huster, W.,
Shaw, D.,
LoBuglio, A.
(1988). Effect of commercial immunoglobulin G preparation on human monocyte Fc-receptor dependent binding of antibody coated platelets. British Journal of Haematology, 68(1), 47-51.
Available at:
https://ecommons.aku.edu/eastafrica_fhs_mc_haematol_oncol/145
Comments
This work was published before the author joined Aga Khan University.