Performance evaluation of ion exchange and affinity chromatography for HbA(1c) estimation in diabetic patients with HbD: A study of 129 samples

Document Type

Article

Department

Pathology and Microbiology

Abstract

Objectives:

To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in Patients with type I and II diabetes having hemoglobin D. Design And

Methods:

Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance forHbA(1c) estimation in Patients with homozygous and heterozygous D disease.

Results:

Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic Patients for HbA(1c) estimation.

Conclusion:

The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.

Publication (Name of Journal)

Clinical Biochemistry

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