Metabolomics of a neonatal cohort from the Alliance for Maternal and Newborn Health Improvement biorepository: Effect of preanalytical variables on reference intervals

Authors

Lena Jafri, Aga Khan UniversityFollow
Aysha Habib Khan, Aga Khan UniversityFollow
Muhammad Ilyas, Aga Khan University
Muhammad Imran Nisar, Aga Khan UniversityFollow
Javairia Khalid, Aga Khan UniversityFollow
Hafsa Majid, Aga Khan UniversityFollow
Aneeta Hotwani, Aga Khan UniversityFollow
Fyezah Jehan, Aga Khan UniversityFollow

Document Type

Article

Department

Paediatrics and Child Health; Pathology and Laboratory Medicine

Abstract

Background: The study was conducted to determine reference interval (RI) and evaluate the effect of preanalytical variables on Dried blood spot (DBS)-amino acids, acylcarnitines and succinylacetone of neonates.
Methodology: DBS samples were collected within 48-72 hours of life. Samples were analyzed for biochemical markers on tandem mass spectrometer at the University of Iowa. Comparison of RI across various categorical variables were performed.
Results: A total of 610 reference samples were selected based on exclusion criteria; 53.2% being females. Mean gestational age (GA) of mothers at the time of delivery was 38.7±1.6 weeks; 24.5% neonates were of low birth weight and 14.3% were preterm. Out of the total 610 neonates, 23.1% were small for GA. Reference intervals were generated for eleven amino acids, thirty-two acylcarnitines and succinylacetone concentrations. Markers were evaluated with respect to the influence of gender, GA, weight and time of sampling and statistically significant minimal differences were observed for some biomarkers.
Conclusion: RI for amino acids, succinylacetone and acylcarnitine on DBS has been established for healthy neonates, which could be of use in the clinical practice. Clinically significant effect of GA, weight, gender and time of sampling on these markers were not identified.

Comments

Pagination are not provided by the author/publisher

Publication (Name of Journal)

PLoS ONE

Recommended Citation

Jafri, L., Khan, A. H., Ilyas, M., Nisar, M. I., Khalid, J., Majid, H., Hotwani, A., Jehan, F. (2023). Metabolomics of a neonatal cohort from the Alliance for Maternal and Newborn Health Improvement biorepository: Effect of preanalytical variables on reference intervals. PLoS ONE, 18(1).
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_pathol_microbiol/1435