Effectiveness of methylene blue photosensitizer-loaded chitosan and sepiolite nanoparticles and Er, Cr: YSGG laser on the smear layer and push-out bond strength of glass fiber post to canal dentin: An in vitro SEM evaluation
Document Type
Article
Department
Community Health Sciences
Abstract
Aim: To investigate the impact of different post space sterilants, namely methylene blue (MB) photosensitizer (PS)-loaded chitosan nanoparticles (CHNPs), sepiolite nanoparticles (SepNPs), and Er, Cr: YSGG laser (ECL), on the smear layer (SL) removal and push-out bond strength (PBS) of glass fiber posts (GFPs) to canal dentin.
Methods: A total of 52 single-rooted premolars were carefully selected, crowns were decoronated, and endodontic therapy was performed; subsequently, the samples were prepared for post space utilizing Gates-Glidden instruments. The specimens were categorized into four cohorts based on the disinfection regime (n = 13): Group 1: 2.5% NaOCl + ethylenediamine tetraacetic acid (EDTA), Group 2: MB-CHNPs-PDT + EDTA, Group 3: MB-SepNPs-PDT + EDTA, and Group 4: ECL + EDTA. SL removal analysis and PS-loaded nanoparticle characterization were executed using scanning electron microscopy. Self-cure resin cement was used for bonding GFP, followed by thermocycling. The evaluation of bond strength and the analysis of the fracture mode were conducted utilizing a universal testing machine and stereomicroscope, respectively. Data analysis was conducted using Analysis of Variance (ANOVA) and a post hoc test (p < 0.05).
Result: The cervical section of Group 2 (MB-CHNPs-PDT + EDTA) test specimens displayed the maximum cleaning of SL (1.22 ± 0.23) and the highest PBS (11.23 ± 0.12 MPa). However, the apical third of Group 1 (MB-SepNPs-PDT + EDTA) reported minimum SL removal (4.53 ± 0.17) and the lowest bond strength (6.65 ± 0.13 MP). Group 1 (2.5% NaOCl + EDTA) and Group 4 (ECL + EDTA) presented no significant variation in SL elimination and PBS of GFP
Conclusion: Using EDTA co-application, the MB-loaded CHNP protocol was associated with lower smear layer scores and higher PBS compared with the other tested groups.
AKU Student
no
Publication (Name of Journal)
Photobiomodulation, Photomedicine, and Laser Surgery
DOI
10.1177/25785478251400605
Recommended Citation
Alanazi, A. M.,
Tahir, R.,
Ishrat, M.,
Zaib, J.,
Abrar, E.
(2026). Effectiveness of methylene blue photosensitizer-loaded chitosan and sepiolite nanoparticles and Er, Cr: YSGG laser on the smear layer and push-out bond strength of glass fiber post to canal dentin: An in vitro SEM evaluation. Photobiomodulation, Photomedicine, and Laser Surgery, 44(1).
Available at:
https://ecommons.aku.edu/pakistan_fhs_mc_chs_chs/1267
Comments
Pagination is not provided by author/publisher.