Dysregulation of metalloproteins in ischemic heart disease patients with systolic dysfunction
Document Type
Article
Department
Biological and Biomedical Sciences; Medicine; Surgery
Abstract
Ischemic heart disease (IHD) is the leading cause of mortality worldwide. Metalloproteins have been linked to human health and diseases. The molecular functions of metalloproteins in IHD is not well understood and require further exploration. The objective of this study was to find out the role of metalloproteins in the pericardial fluid of IHD patients having normal (EF > 45) and impaired (EF < 45) left ventricular ejection fraction (LVEF). IHD patients were grouped into two categories: LVEF45 (n = 33). Pooled samples of pericardial fluid were fractionated by using ZOOM-isoelectric focusing (IEF) followed by further processing using one-dimensional gel electrophoresis (1D SDS-PAGE) and filter-aided sample preparation (FASP). Tryptic peptides of each fraction and differential bands were then analyzed by nano-LC-ESI-MS/MS. Protein identification was performed through a Mascot search engine using NCBI-Prot and SwissProt databases. A total of 1082 proteins including 154 metalloproteins were identified. In the differential bands, 60 metalloproteins were identified, while 115 metalloproteins were identified in all ZOOM-IEF fractions. Twelve differentially expressed metalloproteins were selected in the intense bands according to their molecular weight (MW) and isoelectric point (pI). The 12 differentially expressed metalloprotein includes ceruloplasmin, Prothrombin, Vitamin K-dependent protein, Fibulin-1, Ribosomal protein S6 kinase alpha-6, nidogen, partial, Serum albumin, Hemopexin, C-reactive protein, Serum amyloid P-component, and Intelectin-1 protein which were all up-regulated while serotransferrin is the only metalloprotein that was down-regulated in impaired (LVEF
Publication (Name of Journal)
International Journal of Biological Macromolecules
Recommended Citation
Khan, N.,
Ullah, J.,
Hashmi, S.,
Ali, A.,
Siddiqui, A. J.,
Sami, S. A.,
Bukhari, S. S.,
Sharif, H.,
Uddin, J.,
El-Seedi, H. R.
(2023). Dysregulation of metalloproteins in ischemic heart disease patients with systolic dysfunction. International Journal of Biological Macromolecules, 232.
Available at:
https://ecommons.aku.edu/pakistan_fhs_mc_bbs/996
Comments
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