Protein kinase C inhibitor, chelerythrine, potentiates the adrenaline-mediated aggregation of human platelets through calcium influx

Document Type

Article

Department

Biological and Biomedical Sciences

Abstract

The role of protein kinase C (PKC) using its selective inhibitor, chelerythrine, in agonist mediated platelet aggregation was studied. Chelerythrine had no effect on the aggregation induced by adrenaline, PAF, collagen and ADP at the maximum doses of these agonists. However, it potentiated the aggregatory response of low doses of adrenaline (0.4-1 microM). Such an effect was blocked by Ca(++)-channel blockers, verapamil and diltiazem indicating the likely involvement of Ca++ influx in the platelet aggregation during the cascade.

Publication (Name of Journal)

Biochemistry and Molecular Biology International

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