Generation of a ZEB2 deficient human iPSC line (KICRi002A-4)
Document Type
Article
Department
Biological and Biomedical Sciences
Abstract
The transcription factor ZEB2 is essential for early embryonic development. Using CRISPR/Cas9, we generated a ZEB2 deficient human iPSC cell line (KICRi002A-4), carrying a homozygous 790 bp deletion in ZEB2 that involves part of exon 5, intron 5 and part of exon 6. The deletion leads to markedly reduced levels of a truncated ZEB2 transcript. No ZEB2 protein was detected by immunopreciptation. The iPSC line expressed pluripotency markers and showed a capacity to differentiate into the three germ layers in vitro. Assessment of genomic integrity revealed a normal karyotype without detectable OFF-target editing. The iPSC line KICRi002A-4 thus offers a valuable resource to study the role of ZEB2 for the commitment and differentiation of various human cell lineages.
Publication (Name of Journal)
Stem Cell Research
DOI
10.1016/j.scr.2024.103521
Recommended Citation
Schuster, J.,
Fatima, A.,
Papadopoulos, N.,
Guidi, C. d.,
Sobol, M.,
Dahl, N.
(2024). Generation of a ZEB2 deficient human iPSC line (KICRi002A-4). Stem Cell Research, 80.
Available at:
https://ecommons.aku.edu/pakistan_fhs_mc_bbs/1064
Comments
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