Morphological changes in the rabbit ovary after active immunization to testosterone

Document Type

Article

Department

Paediatrics and Child Health (East Africa)

Abstract

The role of testosterone in follicular development was investigated by immunizing female rabbits (mean wt 1.4 kg) to testosterone-3-bovine serum albumin (T-3-BSA). Controls received BSA. Follicular diameters and histology, and ovarian and uterine weights were recorded at intervals up to 11 weeks. At 5 weeks T-3-BSA ovaries did not differ from controls in either histology or follicular diameter (number of follicles greater than or equal to 1.0 mm were 12.5 +/- 3.4 and 17.0 +/- 1.0 for BSA and T-3-BSA treated animals respectively). By 8 weeks T-3-BSA animals had multiple cystic and hemorrhagic follicles. T-3-BSA ovaries contained more follicles greater than or equal to 1.0 less than 1.5 mm (27.3 +/- 3.1 vs. 15.3 +/- 2.9, p less than 0.01) and greater than or equal 1.5 mm diameter (5.8 +/- 1.7 vs. 0.4 +/- 0.3, p less than 0.005). At 11 weeks T-3-BSA ovaries contained more follicles less than 1.5 mm in diameter (5.0 +/- 0.9 vs. 0.9 +/- 0.3, p less than 0.001). Increased vascularization, some thecal hypertrophy and marked interstitial cell hypertrophy were characteristic of the T-3-BSA ovaries at 8 and 11 weeks. These results suggest that testosterone has a role in the regulation of follicular development.

Comments

This work was published before the author joined Aga Khan University.

Publication (Name of Journal)

Cell and Tissue Research

Share

COinS