eCommons@AKU

Title

Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture

Authors

Lennert Steukers, Laboratory of Virology, Belgium
Steven Weyers, Department of Obstetrics and Gynecology
Xiaoyun Yang, Laboratory of Virology
Annelies P. Vandekerckhove, Laboratory of Virology
Sarah Glorieux, Laboratory of Virology
Maria Cornelissen, Ghent University Hospital, Belgium
Wim Van den Broeck, Ghent University, Belgium
Marleen Temmerman, Aga Khan UniversityFollow
Hans J. Nauwynck, Laboratory of Virology

Document Type

Article

Department

Obstetrics and Gynaecology (East Africa)

Abstract

We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.

Comments

This work was published before the author joined Aga Khan University.

Publication ( Name of Journal)

The Journal of Infectious Diseases

Recommended Citation

Steukers, L., Weyers, S., Yang, X., Vandekerckhove, A. P., Glorieux, S., Cornelissen, M., Broeck, W. V., Temmerman, M., Nauwynck, H. J. (2014). Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture. The Journal of Infectious Diseases, 210(2), 209-213.
Available at: https://ecommons.aku.edu/eastafrica_fhs_mc_obstet_gynaecol/516