Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture
Obstetrics and Gynaecology (East Africa)
We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.
Publication ( Name of Journal)
The Journal of Infectious Diseases
Vandekerckhove, A. P.,
Broeck, W. V.,
Nauwynck, H. J.
(2014). Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture. The Journal of Infectious Diseases, 210(2), 209-213.
Available at: https://ecommons.aku.edu/eastafrica_fhs_mc_obstet_gynaecol/516