Generation of a human anti-idiotypic antibody that mimics the GD2 antigen.
Document Type
Article
Department
Haematology and Oncology, East Africa
Abstract
In a phase 1 trial, patients with metastatic melanoma received the anti-GD2 murine mAb 14G2a. All patients developed human anti-14G2a antibodies including anti-Id antibodies. Peripheral blood MNCs from one such patient were fused with the murine myeloma cell line Ag8. Four human anti-14G2a secreting hybridomas were generated and the mAb product of one of the hybridomas was characterized. The human mAb 4B5 (hu-IgG, lambda) binds to the variable region of murine 14G2a (anti-Id). The 4B5 binds to the antigen-combining site of 14G2a and inhibits its binding to GD2 expressing Mel-21 cells. Rabbits were immunized with the human anti-Id 4B5. Sera from the immunized rabbits demonstrated anti-4B5 antibodies and anti-Mel-21 and anti-GD2 reactivity. Furthermore, rabbit sera competitively inhibited binding of 14G2a to Mel-21 cells. Rabbits immunized with 4B5 developed a DTH response when challenged with 4B5 antibody and Mel-21 cells. These studies demonstrate that the human anti-Id 4B5 mimics the GD2 antigen and is capable of eliciting both a humoral and cellular anti-GD2 immune response. This antibody could be potentially used as a human anti-Id vaccine in patients with malignant melanoma.
Publication (Name of Journal)
The Journal of Immunology
DOI
https://doi.org/10.4049/jimmunol.151.6.3390
Recommended Citation
Saleh, M.,
Stapleton, J. D.,
Khazaeli, M. B.,
Lobuglio, A. F.
(1993). Generation of a human anti-idiotypic antibody that mimics the GD2 antigen.. The Journal of Immunology, 151(6), 3390-3398.
Available at:
https://ecommons.aku.edu/eastafrica_fhs_mc_haematol_oncol/131
Comments
This work was published before the author joined Aga Khan University.