Characteristics and classification of high-grade B-cell lymphomas in resource-limited settings

Document Type



Pathology (East Africa)


The aim of this study is to confirm the guild line of pathological diagnosis of BMFS.

Materials and method:

The registered 1000 cases of Japanese Pediatric MDS/AA central review system were reviewed by board members and used in this study. Bone marrow biopsy and clot materials of paraffin sections were used for immunohistochemistry (IHC).


Aplastic anemia cases showed very severe hypoplastic marrow without megakaryocyte and scarce granulopoiesis. Lymphocytes, especially CD8 þ T-cells, were predominant. RCC cases were hypoplastic marrow with patchy pattern. Ery- thropoiesis were mainly composed by large erythroblasts with left shift and baring hemoglobin fetal type (HbF). A few marrow cells were stained p53. Megakaryocytes with single small nuclei or micromegakaryocytes were demonstrated by IHC. RCMD was hypo-normocellular marrow and distinctive erythropoiesis with left shift. P53 þ cells were relatively more than RCC. RAEB or AML with MRC had increased number of p53 þ cells and CD34 þ blasts. Small and micromegakaryocytes were frequently observed. No evidence of AA or MDS cases were categorized into secondary dyshematopoiesis of unknown causes or inherited BMFS.

Conclusion: Bone marrow pathology with IHC were useful for the definite diagnosis of childhood BMFS.


This work was published before the author joined Aga Khan University.