Brain Derived Neurotrophic Factor (BDNF) is a brain protein implicated in learning, memory and other cognitive functions. Changes in cellular brain functions as well as cognitive defects have been observed the days following anaesthesia, even for short-duration anaesthesia with/without surgery. Despites the role of neurotrophic factors in cognition, no data are still available on brain effects after anaesthesia. Purpose: To study the effect of minor surgery under short duration anaesthesia on cognition by investigating BDNF levels in plasma, hippocampus and cortex. METHODS: Male rats received an intra-peritoneal injection of either 120 mg/kg of propofol or intralipids solution or minor surgery was performed under propofol anaesthesia. The animals were euthanized at ZT5 (peak of the circadian profile of brain BDNF in rat) after 3 days and brain homogenates of prefrontal Cortex and Hippocampus were prepared and blood was also collected for plasma. The amount of BDNF was assessed using ELISA (Millipore) on supernatant.
Rafiq, Muhammad; Mahmood, Zahid; Ali, Sajed; and Pain, Laure
"Effect of minor surgery under propofol anaesthesia on brain BDNF and cognition,"
Pakistan Journal of Neurological Sciences (PJNS): Vol. 13
, Article 3.
Available at: https://ecommons.aku.edu/pjns/vol13/iss1/3