A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan: HBV genotypes in pakistan.

Document Type



Pathology and Microbiology


Background:Hepatitis B virus (HBV) genotypes and mutations are gaining importance in determining the clinical course of chronic liver disease.


To determine and compare the distribution of HBV genotypes and genomic variations in Pakistan to other parts of the world. PatientS and


We conducted a prospective study at Aga Khan University Hospital from December 2006 to December 2008. HBV genotype was determined in 257 HBV DNA-positive Patients. Patients were divided into two groups according to HBeAg positivity. Mutations in the pre-core and core promoter regions of HBV were determined in HBeAg-negative Patients by line probe INNOLIPA assay.


The meanSD age of Patients was 285 years, there were 201 (78%) men. HBeAg was positive in 219 (85%) Patients and negative in 38 (15%). HBeAg-positive Patients were younger than HBeAg-negative Patients (95% vs 21% in ≤30 years, pConclusion: HBV genotype D is predominant in Pakistan, irrespective of HBeAg status. PC and BCP mutations were found in significant numbers of Patients infected with genotype D. The HBV genotype D isolates from Pakistan are identical to the sequences isolated from other parts of the world.

Publication (Name of Journal)

Hepatitis Monthly