Second messenger up-regulation of Androgen Receptor gene transcription is absent in Androgen insensitive human Prostatic Carcinoma cell lines, PC-3 and DU-145
Pathology and Microbiology
A theoretical pathway of transcriptional regulation of the androgen receptor (AR) gene is via a cAMP response element (CRE) present in its promoter region (-508 to -501). After 20 h of stimulation with 8-bromo-cAMP, AR mRNA was upregulated in LNCaP but not in either PC-3 or DU-145 cell lines. We have demonstrated that the level of CRE binding protein (CREB) was the same in all cell lines and that the putative AR-CRE forms specific and compatible protein interactions with CREB. The ability to regulate AR gene transcription via the second messenger pathway is lost in the PC-3 and DU-145 cell lines. This may be an important primary mechanism of androgen insensitivity in prostate cancer.
Stubbs, A. P.,
Stamp, G. W.,
(1996). Second messenger up-regulation of Androgen Receptor gene transcription is absent in Androgen insensitive human Prostatic Carcinoma cell lines, PC-3 and DU-145. Febs Letters, 383(3), 237-240.
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_pathol_microbiol/386