Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ.
Pathology and Microbiology
The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-α stimulation, which induces NF-κB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-κB transcription factor subunits are located in the 5′-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-κB binding and thus regulation of TFF3 expression.
Al-azzeh, E., Romanska, H., Abel, P. D., Kayademir, T., Blin, N., Stamp, G. W., & Lalani, E. (2004). Transcriptional control of TFF3 (intestinal trefoil factor) via promotor binding sites for the nuclear factor kappaB and C/EBPbeta. Peptides, 25(5), 849-854.