Pathology and Laboratory Medicine
In December 2016 physicians in Karachi, Pakistan,witnessed an increase in patients presenting with febrile illness and severe polyarthralgia. Subsequently, chikungunya virus (CHIKV)) was isolated from three patients. This virus was sequenced and compared with other isolates of CHIKV obtained in India and Pakistan during recent outbreaks. Phylogenetic analysis indicated that the Karachi isolates were most similar to the East Central South African CHIKV lineage and showed sequence homology to isolates obtained in other parts of Pakistan and India. More importantly, two of the CHIKV isolates had a nucleotide substitution in the E1 gene corresponding to an amino acid change at chain F portion of the E1 protein.
JPMA. The Journal of the Pakistan Medical Association
(2021). Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates. JPMA. The Journal of the Pakistan Medical Association, 71(5), 1467-1471.
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_pathol_microbiol/1336