Vitamin E inhibits apoptosis of human CD34+-derived erythroid progenitor cells obtained from healthy adults

Document Type

Article

Department

Biological and Biomedical Sciences; Pathology and Laboratory Medicine; Community Health Sciences

Abstract

Inhibition of proapoptotic proteins by various intra- and extracellular factors have been shown to cause increased survival of EPCs. The aim of the present study was to investigate the effect of vitamin E on Tumor Necrosis Factor-Alpha (TNF-a)-induced apoptosis of CD34+- derived EPCs of healthy human adults. CD34+- derived EPCs were isolated from human Peripheral Blood Mononuclear Cells (PBMNCs) and treated in vitro with various concentrations of TNF-a to determine the dose inducing maximum apoptosis. EPCs were incubated at various concentrations of vitamin E or Erythropoietin (EPO) as control for 4 hours before adding 100 ng/mL of TNF-a (a dose producing maximum apoptosis). After 24-hour incubation, quantification of percentage of EPCs undergoing apoptosis was carried out by FITC-Annexin V and PI staining followed by flow cytometry. In a dose-dependent manner, TNF-a alone increased the percentage of early and late apoptosis of treated CD34+- derived EPCs. There was a significant difference in the mean percentage of TNF-a-induced apoptotic CD34+-derived EPCs in early and late apoptosis by vitamin E treatment (p value= 0.080) which was higher in early as compared to late apoptosis. There was a significant difference in the percentages of TNF-a induced early and late apoptosis of CD34+- derived EPCs by vitamin E treatment or EPO treatment when analyzed for a statistical interactive effect on early and late apoptosis and drug concentration (p value < 0.001).The study suggests a positive role of vitamin E in decreasing TNF-a induced apoptosis of CD34+- derived EPCs from healthy human adults.

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Pagination are not provided by the author/publisher

Publication (Name of Journal)

Annals of Hematology and Oncology

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