Analysis of anti-microbial and anti-biofilm activity of hand washes and sanitizers against S. aureus and P. aeruginosa
Document Type
Article
Department
Biological and Biomedical Sciences
Abstract
Objective: To analyse the biofilm-forming potential of clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa, and to assess antimicrobial activity of commonly used sanitizers in hospital and laboratory settings.
Methods: The study was conducted at Aga Khan University Karachi from August 2016 to January 2017. The biofilm-forming potential of Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates were evaluated qualitatively using air-liquid interface tube method, and air-liquid interface cover slip assay. The antimicrobial activity of commonly-used hand-washes and sanitizers were assessed using agar well diffusion method, while the anti-biofilm activity of the hand-washes and sanitizers was qualitatively assessed using air-liquid interface covers lip as s ay.
Results: Of the eight hand-washes and sanitizers, 2(25%) showed antimicrobial activity against both Staphylococcus aureus and Pseudomonas aeruginosa, while 2(25%) exhibited antimicrobial activity against either S. aureus or P. aeruginosa. Also, 4 (50%) of them showed no inhibitory activity against S. aureus and P. aeruginosa.
Conclusions: The findings shall have important consequences with regards to infection control in hospital and laboratory settings.
Publication (Name of Journal)
JPMA. The Journal of the Pakistan Medical Association
Recommended Citation
Ahmed, K.,
Ahmed, H.,
Ahmed, F. A.,
Ali, A. A.,
Akbar, J.,
Rana, J.,
Tariq, U.,
Abidi, S. H.
(2020). Analysis of anti-microbial and anti-biofilm activity of hand washes and sanitizers against S. aureus and P. aeruginosa. JPMA. The Journal of the Pakistan Medical Association, 70(1), 100-104.
Available at:
https://ecommons.aku.edu/pakistan_fhs_mc_bbs/807