Biological and Biomedical Sciences
Objectives: Non-Steroidal anti-inflammatory drugs (NSAIDS) have long been used as anti-inflammatory agents, yet their mode of action is not entirely clear. The inhibitory effects of NSAIDS on prostaglandin production can only partly explain their anti-inflammatory actions. This study was aimed at defining the role of cycl-oxygenase (COX) inhibitors on nitric oxide (NO) production in murine macrophages in vitro.
Methods: Murine macrophages were obtained from the peritoneum and after exposure, in vitro to lipopolysaccharide (LPS) produced nitrite, measured after 24 hours by Griess reaction. The macrophages were pre-incubated with aspirin or indomethacin before activation with LPS.
Results: Treatment with aspirin resulted in an increase in nitric oxide production. A similar response was obtained with indomethacin treatment.
Conclusion: This study shows that COX inhibitors significantly increase NO production in murine macrophages in vitro and this may be one of the mechanisms by which they exert their anti-inflammatory effects.
Publication ( Name of Journal)
Journal of Pakistan Medical Association
Zuberi, H. S.,
Ashfaq, M. K.
(2001). Nitric oxide mediated effect of cyclo-oxygenase inhibitors. Journal of Pakistan Medical Association, 51(1), 28-31.
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_bbs/571