Biological and Biomedical Sciences
N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay.
Journal of Pakistan Medical Association
Javed, M. H.,
Hussain, A. N.,
Waqar, M. A.
(1992). Estimation and separation of N-acetyl-beta-D-glucosaminidase isoenzymes in urine. Journal of Pakistan Medical Association, 42(3), 64-66.
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_bbs/479