Biological and Biomedical Sciences
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
Experimental and Molecular Medicine
Javed, M. U.,
Waqar, S. N.
(2001). An enzymatic method for the detection of human serum albumin. Experimental and Molecular Medicine, 33(2), 103-105.
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