A sensitive enzyme-linked immunosorbent assay for dihydrofolate reductase
Biological and Biomedical Sciences
A competitive enzyme-linked immunosorbent assay (ELISA) has been developed for the enzyme, dihydrofolate reductase. The sensitivity of the assay system approximates 3 ng of immunoreactive enzyme protein which is comparable to the sensitivity achieved in the radioimmunoassay (RIA) for this enzyme. The mean coefficient of variation for within--and between--assay precision was less than 13%. The assay appears to be specific and valid as the concentration of the enzyme is the same whether measured by ELISA or [3H]-methotrexate binding. Since this method, like the RIA, measures the mass concentration of enzyme protein, in conjunction with a [3H]-methotrexate binding assay it will be useful for quantitating functional as well as non-functional immunoreactive forms of the enzyme.
The Italian Journal of Biochemistry
Iqbal, M. P.,
(1991). A sensitive enzyme-linked immunosorbent assay for dihydrofolate reductase. The Italian Journal of Biochemistry, 40(4), 207-215.
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