Document Type
Article
Department
Biological and Biomedical Sciences
Abstract
Simian virus 40 large T-antigen (SV40 LT-Ag) is a 708 amino acid nuclear phosphoprotein. Among many functions of LT-Ag is its ability to perform as an ATPase-helicase, catalyzing the unwinding of viral genome during replication. The LT-Ag has been employed in the studies of helicase structure and function, and has served as a model helicase for the screening of antiviral drugs that target viral helicase. In this study, using in vitro enzyme assays and in silico computer modeling, we screened a batch of 18 fluoroquinolones to assess their potential as antivirals by virtue of their inhibition of the LT-Ag helicase. We found all fluoroquinolones to be inhibitory to the helicase activity of LT-Ag. In our docking analysis, most of these tested drugs showed similarity in their interactions with LT-Ag. Our study shows the potential of fluoroquinolones as antiviral drugs and of SV40 LT-Ag as a model protein for screening drugs against viral helicases.
Publication (Name of Journal)
Bioinformation
Recommended Citation
Siddiqui, S.,
Anwar, M. F.,
Naeem, S.,
Abidi, S. H.,
Zarina, S.,
Ali, S.
(2018). Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases. Bioinformation, 14(2), 75-79.
Available at:
https://ecommons.aku.edu/pakistan_fhs_mc_bbs/343
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This work is licensed under a Creative Commons Attribution 4.0 International License.
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Amino Acids, Peptides, and Proteins Commons, Biological Factors Commons, Biology Commons