The purification of α1-antichymotrypsin from human serum using DNA-cellulose chromatography
Biological and Biomedical Sciences
By exploiting its capacity for binding to DNA, the protease inhibitor α1-antichymotrypsin has been isolated from human serum by ammonium sulfate fractionation and successive chromatography on QAE-Sephadex, DNA-cellulose, and Sephacryl S-300. This experimental procedure compares favorably with existing methods for preparing α1-antichymotrypsin in terms of overall yield and practical convenience. The purified α1-antichymotrypsin was homogeneous as judged by electrophoretic and immunoelectrophoretic criteria. From its inhibition of the fluorimetric titration of chymotrypsin with 4-methylumbelliferyl-p-trimethylammonium cinnamate it was shown to combine with chymotrypsin in a 1:1 molar ratio and thus to retain its biological activity
Archives of Biochemistry and Biophysics
Siddiqui, A. A.,
Hill, J. A.,
(1983). The purification of α1-antichymotrypsin from human serum using DNA-cellulose chromatography. Archives of Biochemistry and Biophysics, 225(1), 306-312.
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