Sperm motility characteristics and oxidative stress in crude oil exposed rats

Document Type



Biomedical Sciences (East Africa)


OBJECTIVE: Evaluation of micro-environment is a surrogate mean for oocyte quality assessment. This study tested the hypothesis that an association exists between follicular fluid trace elements and IVF outcomes. DESIGN: Prospective Cohort study.

MATERIALS AND METHODS: Through one year, women attending a university-affiliated IVF center were counseled to participate in our study. FF from large follicles; 17 mm or more, containing only one follicle was eligible for sampling. samples centrifugated at 1500 rpm for 5 minutes and the supernatants were stored at - 80 till IVF results were disclosed. Samples were categorized into 2 groups; group 1 included pregnant women, while group 2 samples from non-pregnant women. Twenty-five women samples were randomly selected from the consecutively frozen samples in each group for trace element analysis. Cadmium, lead, and Zinc was measured. The research randomizer (www.randomizer.org) used. Correlation and regression analysis were done to assess the relation between the studied trace elements and IVF outcome.

RESULTS: 25 pregnant and 24 non-pregnant women samples were analyzed. Both groups were comparable in age, BMI, AMH, antral follicle count, infertility duration and IVF indications. Non-pregnant women show less number of retrieved and fertilized oocytes than pregnant women (median and 5P0.05 and 1.2vs1.5. A significant Positive correlation was found between zinc concentrations and occurrence of pregnancy.

CONCLUSIONS: In addition to the quality of embryos, maintenance of higher zinc in FF seems to be essential for favorable IVF outcome.

Publication (Name of Journal)

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