Document Type
Article
Department
Brain and Mind Institute
Abstract
INTRODUCTION Brain-derived extracellular vesicles (BEVs) in blood allows for minimally-invasive investigations of central nervous system (CNS) -specific markers of age-related neurodegenerative diseases (NDDs). Polymer-based EV- and immunoprecipitation (IP)-based BEV-enrichment protocols from blood have gained popularity. We systematically investigated protocol consistency across studies, and determined CNS-specificity of proteins associated with these protocols.
METHODS NDD articles investigating BEVs in blood using polymer-based and/or IP-based BEV enrichment protocols were systematically identified, and protocols compared. Proteins used for BEV-enrichment and/or post-enrichment were assessed for CNS- and brain-cell-type-specificity, extracellular domains (ECD+), and presence in EV-databases.
RESULTS A total of 82.1% of studies used polymer-based (ExoQuick) EV-enrichment, and 92.3% used L1CAM for IP-based BEV-enrichment. Centrifugation times differed across studies. A total of 26.8% of 82 proteins systematically identified were CNS-specific: 50% ECD+, 77.3% were listed in EV-databases.
CONCLUSIONS We identified protocol steps requiring standardization, and recommend additional CNS-specific proteins that can be used for BEV-enrichment or as BEV-biomarkers.
Publication (Name of Journal)
Alzheimer's & Dementia
DOI
https://doi.org/10.1002/alz.13823
Recommended Citation
Badhwar, A.,
Hirschberg, Y.,
Valle-Tamayo, N.,
Iulita, F.,
Momoh, C.,
Matton, A.,
Tarawneh, R.,
Rissman, R.,
Ledreux, A.,
Winston, C.
(2024). Assessment of brain-derived extracellular vesicle enrichment for blood biomarker analysis in age-related neurodegenerative diseases: An international overview. Alzheimer's & Dementia, 20(7), 4411-4422.
Available at:
https://ecommons.aku.edu/bmi/433
Creative Commons License
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