A study of genotypes, mutants and nucleotide sequence of hepatitis B virus in Pakistan: HBV genotypes in pakistan.

Document Type

Article

Department

Pathology and Microbiology

Abstract

Background:Hepatitis B virus (HBV) genotypes and mutations are gaining importance in determining the clinical course of chronic liver disease.

Objectives:

To determine and compare the distribution of HBV genotypes and genomic variations in Pakistan to other parts of the world. PatientS and

Methods:

We conducted a prospective study at Aga Khan University Hospital from December 2006 to December 2008. HBV genotype was determined in 257 HBV DNA-positive Patients. Patients were divided into two groups according to HBeAg positivity. Mutations in the pre-core and core promoter regions of HBV were determined in HBeAg-negative Patients by line probe INNOLIPA assay.

Results:

The meanSD age of Patients was 285 years, there were 201 (78%) men. HBeAg was positive in 219 (85%) Patients and negative in 38 (15%). HBeAg-positive Patients were younger than HBeAg-negative Patients (95% vs 21% in ≤30 years, pConclusion: HBV genotype D is predominant in Pakistan, irrespective of HBeAg status. PC and BCP mutations were found in significant numbers of Patients infected with genotype D. The HBV genotype D isolates from Pakistan are identical to the sequences isolated from other parts of the world.

Publication (Name of Journal)

Hepatitis Monthly

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