Title

Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ.

Document Type

Article

Department

Pathology and Microbiology

Abstract

The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-α stimulation, which induces NF-κB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-κB transcription factor subunits are located in the 5′-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-κB binding and thus regulation of TFF3 expression.

Publication

Peptides