Title

Helicobacter pylori-induced lipid peroxidation in peripheral blood lymphocytes

Document Type

Article

Department

Medicine; Gastroenterology

Abstract

Several lines of evidence implicate Helicobacter pylori infection in the pathogenesis of gastritis and peptic ulceration. To investigate whether H. pylori can cause lipid peroxidation in lymphocytes in vitro and to look for experimental evidence of lipid peroxidation induced by H. pylori, the lipid peroxide (LPO) level in peripheral blood lymphocytes was measured using the thiobarbituric acid fluorescence method. In the absence of added H. pylori, the LPO level in lymphocytes was 0.133±0.033 nmol/106 cells, and in the co-culture of H. pylori with peripheral blood mononuclear cells 0.340±0.097 nmol/ 106 cells. A significant difference was found between the two groups (p<0.001). Antioxidants, either superoxide dismutase or catalase, could inhibit LPO production in lymphocytes. The present data provide further evidence that H. pylori can induce lipid peroxidation, which may be responsible for the pathogenesis of H. pylori-associated mucosal damage.

Comments

This work was published prior to author joined Aga Khan University

Publication

APMIS