Diversity of Helicobacter pylori among Chinese persons with H. pylori infection.

Authors

Javed Yakoob, Aga Khan UniversityFollow
Guo Ling Hu, Hunan Medical University
Xue Gong Fan, Hunan Medical University
Hui Xiang Yang, Hunan Medical University
De Ming Tan, Hunan Medical University
Tie Gan Li, Hunan Medical University
Zheng Zhang, Hunan Medical University

Document Type

Article

Department

Medicine; Gastroenterology

Abstract

To determine whether there is diversity among clinical isolates of Helicobacter pylori in Chinese patients with peptic ulcer disease, 40 strains of H. pylori were isolated from antral biopsy specimens obtained at the gastroenterology clinic of Xiangya Hospital from January 1996 to June 1998. Total protein profile by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and DNA diversity by polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) fingerprinting were performed with these isolates. All the isolates from peptic ulcer disease were relatively homogeneous in protein profiles, but they showed a great DNA sequence diversity by PCR-RAPD fingerprinting. In Chinese patients H. pylori demonstrated an enormous diversity. The diversity among clinical isolates of H. pylori could be distinctly demonstrated and this observation will be helpful in the management of intrafamilial and recurrent H. pylori infection. PCR-RAPD fingerprinting is an efficient method of distinguishing between clinical isolates of H. pylori.

Comments

This work was published prior to author joined Aga Khan University

Publication (Name of Journal)

APMIS

Recommended Citation

Yakoob, J., Hu, G., Fan, X., Yang, H., Tan, D., Li, T., Zhang, Z. (2000). Diversity of Helicobacter pylori among Chinese persons with H. pylori infection.. APMIS, 108(7-8), 482-486.
Available at: https://ecommons.aku.edu/pakistan_fhs_mc_med_gastroenterol/66