Activity-guided Isolation of a Novel Protein from Croton tiglium with Antifungal and Antibacterial Activities
Biological and Biomedical Sciences
This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH(4))(2)SO(4) precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.
(2008). Activity-guided Isolation of a Novel Protein from Croton tiglium with Antifungal and Antibacterial Activities. Phytotherapy Research, 22(12), 1646-1649.
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