Document Type
Article
Department
Pathology (East Africa)
Abstract
We assessed the influence that consecutive-day blood sampling, compared with single-day blood sampling, had on polymerase chain reaction (PCR)-adjusted parasitological cure after stepwise genotyping of merozoite surface proteins 2 (msp2) and 1 (msp1) in 106 children in Tanzania who had uncomplicated falciparum malaria treated with either sulfadoxine-pyrimethamine or artemether- lumefantrine; 78 of these children developed recurrent parasitemia during the 42-day follow-up period. Initial msp2 genotyping identified 27 and 33 recrudescences by use of single- and consecutive-day sampling, respectively; in subsequent msp1 genotyping, 17 and 21 of these episodes, respectively, were still classified as recrudescences; these results indicate a similar sensitivity of the standard single-day PCR protocol - that is, 82% (27/33) and 81% (17/21), in both genotyping steps. Interpretation of PCR-adjusted results will significantly depend on methodology. © 2007 by the Infectious Diseases Society of America. All rights reserved.
Publication (Name of Journal)
Journal of Infectious Diseases
Recommended Citation
Martensson, A.,
Ursing, J.,
Veiga, M. I.,
Wiklund, L.,
Membi, C.,
Montgomery, S. M.,
Premji, Z.,
Farnert, A.,
Bjorkman, A.,
Ngasala, B.
(2007). Influence of consecutive-day blood sampling on polymerase chain reaction-adjusted parasitological cure rates in an antimalarial-drug trial conducted in Tanzania. Journal of Infectious Diseases, 195(4), 597-601.
Available at:
https://ecommons.aku.edu/eastafrica_fhs_mc_pathol/70
Comments
This work was published prior to author’s joining Aga Khan University