Event Title

Relationship of non-esterified fatty acids to lipoproteins and homeostasis model assessment of insulin resistance

Location

Auditorium Pond Side

Start Date

26-2-2014 10:30 AM

Abstract

Objective: To assess the association of non-esterified fatty acids (NEFA) with insulin resistance using homeostasis model assessment of insulin resistance (HOMA-IR), glucose metabolism and dyslipidemias.

Materials and Methods: This study was conducted at the Section of Chemical Pathology, Department of Pathology and Microbiology AKU in September 2013. Specimens of subjects with same day lipid profile and glucose fasting with medical record number were included. Specimens were centrifuged within 4 hours and serum was stored at -80ºC till further analysis. History was retrieved from medical charts. NEFA and insulin were analyzed in batch with controls. Insulin resistance was calculated using homeostasis model assessment of insulin resistance (HOMA-IR).

Results: Mean age of the subjects (n=91) was 49.3±12.7 years, 61.8% being males. 38.5% of the subjects had hypertriglyceridemia along with hypercholesterolemia, 25.3% has hypercholesterolemia, and 9.9% had hypertriglyceridemia. There were 35 subjects (25.3%) with all lipoproteins within the optimal limits. Out of the total study group 36.3% had impaired fasting glucose and 14.3% had fasting glucose in diabetic range. The overall median HOMA-IR was 3.08 (range: 0.75-32.1). HOMA-IR was high (>2) in 78% (n=71) of the subjects. Highest concentration of NEFA (median 0.6 mmol/L; range 0.2-1.2) and HOMA-IR (median 6.75; range 1.2-32.1) were noted in the diabetics. NEFA positively correlated with HOMA-IR (r=0.25,p<0.05), cholesterol (0.28,p<0.05), triglycerides (0.48,p<0.01), glucose fasting (r=0.25,p<0.05), dyslipidemias (r=0.17,p<0.05) and negatively correlated with high density lipoproteins(r=-0.1,p>0.05).There were 29 subjects (31.8%) with both high NEFA and HOMA-IR high and mean comparison showed that all lipoproteins were higher in this group compared to the rest of the subjects.

Conclusion: Our results underline the importance of NEFA as an important therapeutic target in metabolic syndrome.

Keywords: insulin, glucose, fatty acids, diabetes

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Feb 26th, 10:30 AM

Relationship of non-esterified fatty acids to lipoproteins and homeostasis model assessment of insulin resistance

Auditorium Pond Side

Objective: To assess the association of non-esterified fatty acids (NEFA) with insulin resistance using homeostasis model assessment of insulin resistance (HOMA-IR), glucose metabolism and dyslipidemias.

Materials and Methods: This study was conducted at the Section of Chemical Pathology, Department of Pathology and Microbiology AKU in September 2013. Specimens of subjects with same day lipid profile and glucose fasting with medical record number were included. Specimens were centrifuged within 4 hours and serum was stored at -80ºC till further analysis. History was retrieved from medical charts. NEFA and insulin were analyzed in batch with controls. Insulin resistance was calculated using homeostasis model assessment of insulin resistance (HOMA-IR).

Results: Mean age of the subjects (n=91) was 49.3±12.7 years, 61.8% being males. 38.5% of the subjects had hypertriglyceridemia along with hypercholesterolemia, 25.3% has hypercholesterolemia, and 9.9% had hypertriglyceridemia. There were 35 subjects (25.3%) with all lipoproteins within the optimal limits. Out of the total study group 36.3% had impaired fasting glucose and 14.3% had fasting glucose in diabetic range. The overall median HOMA-IR was 3.08 (range: 0.75-32.1). HOMA-IR was high (>2) in 78% (n=71) of the subjects. Highest concentration of NEFA (median 0.6 mmol/L; range 0.2-1.2) and HOMA-IR (median 6.75; range 1.2-32.1) were noted in the diabetics. NEFA positively correlated with HOMA-IR (r=0.25,p<0.05), cholesterol (0.28,p<0.05), triglycerides (0.48,p<0.01), glucose fasting (r=0.25,p<0.05), dyslipidemias (r=0.17,p<0.05) and negatively correlated with high density lipoproteins(r=-0.1,p>0.05).There were 29 subjects (31.8%) with both high NEFA and HOMA-IR high and mean comparison showed that all lipoproteins were higher in this group compared to the rest of the subjects.

Conclusion: Our results underline the importance of NEFA as an important therapeutic target in metabolic syndrome.

Keywords: insulin, glucose, fatty acids, diabetes